翻訳と辞書 Words near each other ・ Hot Springs, California ・ Hot Springs, Lassen County, California ・ Hot Springs, Modoc County, California ・ Hot Springs, Montana ・ Hot Springs, Nevada ・ Hot Springs, North Carolina ・ Hot springs, Pachia ・ Hot Springs, Plumas County, California ・ Hot Springs, South Dakota ・ Hot Springs, Virginia ・ Hot Springs, Washington ・ Hot stain ・ Hot stamping ・ Hot Standby Router Protocol ・ Hot start ・ Hot start PCR ・ Hot Steamy Glasses ・ Hot stick ・ Hot Stove ・ Hot stove league ・ Hot Streak ・ Hot Streak (album) ・ Hot Streets ・ Hot Stuff ・ Hot Stuff (1929 film) ・ Hot Stuff (1956 film) ・ Hot Stuff (1971 film) ・ Hot Stuff (1979 film) ・ Hot Stuff (Donna Summer song) ・ Hot Stuff (Kumi Koda song) Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Hot start PCR ： ウィキペディア英語版 Hot start PCR Hot start PCR is a modified form of Polymerase chain reaction (PCR) which avoids a non-specific amplification of DNA by inactivating the taq polymerase at lower temperature. In this dsDNA is denatured by heating the sample at its denaturing temperature and then the temperature is suddenly reduced to 55℃ at which primer and Taq-polymerase is added, but here the difference arises i.e. specific antibodies are used to block this Taq-polymerase at annealing temperature. Now when the temperature raises for amplification to 72℃, the specific antibody detaches from Taq-polymerase and the amplification with greater specificity starts. In conventional PCR, the Taq DNA polymerase is active at room temperature and to a lesser degree, even on ice. In some instances, when all the reaction components are put together, nonspecific primer annealing can occur due to these low temperatures. This nonspecific annealed primer can then be extended by the Taq DNA polymerase, generating nonspecific products and lowering product yields. Hot Start PCR significantly reduces nonspecific priming, the formation of primer dimers, and often, increases product yields. In Hot Start Long and Accurate PCR, the impact on yield can be dramatic. Classic methods, while effective, involve additional handling and increased risk of contamination. ==References== 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Hot start PCR」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.